Publications

Peer-reviewed

Defining neuronal responses to the neurotropic parasite Toxoplasma gondii.

Johnson HJ,  Kochanowsky JA, Sambamurthy C, Hunter CA, Beiting DP, Koshy AA. (2025). mSphere

 

Immune targeting and host-protective effects of the latent state of Toxoplasma gondii.

Eberhard JN,  Shallberg LA, Winn A, Sambamurthy C, Giuliano CJ, Merritt EF, Willis E, Konradt C, Christian DA, Aldridge DL, Bunkofske ME, Jacquet M, Dzierszinski F, Katifori E, Lourido S, Koshy AA, Hunter CA. (2025). Nature Microbiology

 

Engineering Toxoplasma gondii secretion systems for intracellular delivery of multiple large therapeutic proteins to neurons.

Bracha S, Johnson HJ, Pranckevicius NA, Catto F, Economides AE, Litvinov S, Hassi K, Rigoli MT, Cheroni C,   Bonfanti M, Valenti A, Stucchi S, Atrreya S, Ross PD, Walsh D, Malachi N, Livne H, Eshel R, Krupalnik V, Levin D, Cobb, S, Koumoutsakos P, Caporale N, Test G, Aguzzi A, Koshy AA, Sheiner L, Rechavi O. (2024). Nature Microbiology

 

Toxoplasma type II effector GRA15 has limited influence in vivo.

Merritt EF, Kochanowsky JA, Herve P, Watson AA, Koshy AA. (2024). PLoS One

 

High Throughput identification of Toxoplasma gondii effector proteins that target host cell transcription.

Butterworth S, Kordova K, Sambamurthy C, Thomas KK, Torelli F, Lockyer EJ, Edwards A, Goldstone R, Koshy AA, Treeck M. (2023). Cell Host & Microbe

 

A positive feedback loop controls Toxoplasma chronic differentiation.

Licon MH, Giuliano CJ, Chan AW, Chakladar S, Eberhard JN, Shallberg LA, Sambamurthy C, Waldman BS, Koshy AA, Hunter CA, Lourido S. (2023). Nature Microbiology

 

Understanding neuroinflammation through central nervous system infections.

Johnson HJ, Koshy AA. (2022). Current Opinion in Neurobiology

 

IFN-gamma stimulated murine and human neurons mount anti-parasitic defenses against the intracellular parasite Toxoplasma gondii.

Sambamurthy C, Kochanowsky JA, Merritt EF, Lagas JS, Swannigan A, Koshy AA. (2022). Nature Communications

 

Impact of secondary TCR engagement on the heterogeneity of pathogen-specific CD8⁺ T cell response during acute and chronic toxoplasmosis.

Shallberg LA, Phan AT, Christian DA, Perry JA, Haskins BE, Beiting DP, Harris TH, Koshy AA, Hunter CA. (2022). PLoS Pathogens

 

Injection with Toxoplasma gondii protein affects neuron health and survival.

Mendez OA, Machado EF, Lu J, Koshy AA. (2021). eLife

 

ROP16-Mediated Activation of STAT6 Suppresses Host Cell Reactive Oxygen Species Production, Facilitating Type III Toxoplasma gondii Growth and Survival.

Kochanowsky JA, Thomas KK, Koshy AA. (2021). mBio

 

Transcriptional Profiling Suggests T Cells Cluster around Neurons Injected with Toxoplasma gondii Proteins.

Merritt EF*, Johnson HJ*, Wong ZS, Buntzman AS, Conklin AC, Cabral CM, Romanoski CE, Boyle JP, Koshy AA. (*equal contributors) (2020). mSphere

 

Aging with Toxoplasma gondii results in pathogen clearance, resolution of inflammation, and minimal consequences to learning and memory.

McGovern KE, Cabral CM, Morrison HW, Koshy AA. (2020). Scientific Reports

 

Latent Toxoplasmosis Effects on Rodents and Humans: How Much is Real and How Much is Media Hype?

Johnson HJ, Koshy AA. (2020). mBio

 

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell Responses.

Chen L, Christian DA, Kochanowsky JA, Phan AT, Clark JT, Wang S, Berry C, Oh J, Chen X, Roos DS, Beiting DP, Koshy AA, Hunter CA. (2020). Journal of Experimental Medicine

 

Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions In Situ.

Cabral CM, Merritt EF, Koshy AA. (2020). Methods in Molecular Biology

 

The ROP16_III-Dependent Early Immune Response Determines the Subacute CNS Immune Response and Type III Toxoplasma gondii Survival.

Tuladhar S, Kochanowsky JA, Bhaskara A, Ghotmi Y, Sambamurthy C, Koshy AA. (2019). PLoS Pathogens

 

Semi-automated quantification and neuroanatomical mapping of heterogeneous cell populations.

Mendez OA*, Potter CP*, Valdez M, Bello T, Trouard TP, Koshy AA. (*equal contributors) (2018). Journal of Neuroscience Methods

 

Dissecting Amyloid Beta Deposition Using Distinct Strains of the Neurotropic Parasite Toxoplasma gondii as a Novel Tool.

Cabral CM, McGovern KE, McDonald WR, Franco J, Koshy AA. (2017). ASN Neuro

 

Toxoplasma gondii: Entry, association, and physiological influence on the central nervous system.

Mendez OA, Koshy AA. (2017). PLoS Pathogens

 

STAT1 signaling in astrocytes is essential for control of infection in the central nervous system.

Hidano S, Randall L, Dawson L, Dietrich HK, Konradt C, Klover PJ, John B, Harris TH, Fang Q, Turek B, Kobayashi T, Hennighausen L, Beiting DP, Koshy AA, Hunter CA. (2016). mBio

 

Endothelial cells as a replicative niche for pathway entry to the CNS.

Konradt C, Ueno N, Christian DA, Delong J, Harms-Pritchard G, Herz J, Bzik DJ, Koshy AA, McGavern DB, Lodoen MB, Hunter CA. (2016). Nature Microbiology

 

Neurons are the primary target cell for the brain-tropic intracellular parasite Toxoplasma gondii.

Cabral CM*, Tuladhar S*, Dietrich HK, Nguyen E, MacDonald WR, Trivedi T, Devineni A, Koshy AA. (*equal contributors and listed alphabetically) (2016). PLoS Pathogens

 

Internalization and TLR-dependent type I interferon production by monocytes in response to Toxoplasma gondii.

Han SJ, Melichar HJ, Coombes JL, Chan SW, Koshy AA, Boothroyd JC, Barton GM, Robey EA. (2014). Immunol Cell Biol

 

3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii.

Koshy AA, Cabral CM. (2014).J. Vis. Exp.

 

Toxoplasma gondii development of its replicative niche: in its host cell and beyond.

Blader IJ, Koshy AA. (2014). Eukaryot Cell.

 

The use of transgenic parasites and host reporters to dissect the events that promote IL-12 production during toxoplasmosis.

Christian DA, Koshy AA, Reuter MA, Betts MR, Boothroyd JC, Hunter CA. (2014). Infect Immun.

 

Astrocytic TGF-β signaling fine-tunes the brain’s immune response to Toxoplasma gondii infection.

Cekanaviciute E, Dietrich HK, Axtell RC, Williams AM, Egusquiza R, Wai KM, Koshy AA*, Buckwalter MS*. (*equal contributors) (2014). J. Immunol.

 

Parasite fate and involvement of infected cells in the induction of CD4 and CD8 T cell responses to Toxoplasma gondii.

Dupont CD, Christian DA, Selleck EM, Pepper M, Leney-Greene M, Harms-Pritchard G, Koshy AA, Wagage S, Reuter MA, Sibley LD, Betts MR, Hunter CA. (2014). Plos Pathog.

 

Motile invaded neutrophils in the small intestine of Toxoplasma gondii infected mice reveal a potential mechanism for parasite spread.

Coombs JL, Charsar BA, Han SJ, Halkias J, Chan SW, Koshy AA, Striepen B, Robey EA. (2013). PNAS.

 

A nucleotide sugar transporter (NST1) involved in glycosylation of the Toxoplasma cyst wall is required for efficient persistence of bradyzoite.

Caffaro CE, Koshy AA, Liu L, Zeiner GM, Hirschberg CB, Boothroyd JC. (2013). PLoS Pathogens.

 

Toxoplasma co-opts host cells it does not invade.

Koshy AA, Dietrich, HK, Christian, DA, Melehani, JH, Shastri, AJ, Hunter, CA, Boothroyd, JC. (2012). PLoS Pathogens.

 

Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.

Koshy AA, Fouts, AE, Lodoen, MB, Alkan, O, Blau, HM, Boothroyd, JC. (2010). Nature Methods.